FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649194
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46180.7113436045311571No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38800.5976641805069055No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.4162084061158914No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.3653761433408195No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA23010.3544395049861829No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.3012966848122441No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15220.2344445574050284No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14710.2265886622488809No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.21950295289235575No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA13000.20024830790179826No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.19393278434489536No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.16173901792068318No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA10210.15727194028287383No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA9840.15157256536566882No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACG9180.14140611281065443No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9170.14125207565072997No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.13478251493390267No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA8550.13170177173541348No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA8540.131547734575489No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.13016140013616886No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT8020.12353780225941706No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.12353780225941706No Hit
GTATTAATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTC7920.12199743066017246No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7890.12153531918039909No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7520.11583594426319405No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA7460.11491172130364728No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7450.11475768414372282No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC7350.11321731254447824No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTTGCTTTTCCTCCGCAA7010.10798004910704659No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC6960.10720986330742428No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC6900.10628564034787752No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG6870.10582352886810413No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT6860.10566949170817967No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA6810.1048993059085574No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.10428315726885955No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC6660.10258874850969048No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT6500.10012415395089913No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGC259.1564885E-567.9893953
ATACGGG2600.063.7449721
TGGTATC24650.053.267562
ATATCAA10400.052.712191
AGTATCA3900.052.3035661
AAGTACG5950.052.138751
GTGGTAT24250.051.521711
ACCTAGC250.007794789550.9999059
TAACTCG250.00779716450.9959754
TCTGTCG603.2084245E-642.496658
AGTCGTA450.001662527737.777719
GTGTATA4950.037.7747961
GTATCAA87050.034.661251
TGAGTCG500.00278917533.997327
TAGGCAT500.00278917533.997325
TATCAAC110950.032.1333432
GGGGTAC802.3069055E-531.8724867
GTATAAG6450.031.6229763
GCCGGCT950.031.32055946-47
GGTATCA49000.031.13531
Produced by FastQC (version 0.11.5)