FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685875
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203202.962638964825952No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22740.3315472936030618No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15950.23254966283943868No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12040.17554219063240387No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12010.1751047931474394No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8930.1301986513577547No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGG8670.12640787315472934No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT7800.11372334609075997No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA7320.1067249863313286No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT7080.1032258064516129No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7070.10308000728995809No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC6940.10118461818844542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAT150.005780344455.005472
CCACATG150.005783681354.99744852
CCGTTCT351.5707064E-647.1441146
GCGGTAT403.9033966E-641.30231
GTGGTAT22200.041.30231
TGGTATC22450.040.6721952
GTCGCCC300.001924831236.6676434
AGTAGTC401.9012668E-434.373425
TGTGGTA350.00409649631.43172
ATCGGCG350.004097958631.42940711
CTAGATG350.004099421731.42711421
TGCCTAG350.004100885231.42482418
GGCCGTT553.4881792E-530.00079544
GCCGTTC553.4881792E-530.00079545
GTGTATA4050.029.9144231
TCGGGAT653.20437E-629.6140149
AGCCGTC852.7050191E-829.1184238
GTATAGA606.259847E-527.5348661
GGGCCGT606.312966E-527.5007343
CGGAGAT507.0279173E-427.5007299
Produced by FastQC (version 0.11.5)