FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067806
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76470.7161413215509185No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20200.18917293965383225No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14940.13991305536773535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16650.042.6387981
TGGTATC16850.040.9741972
TCGAGTC300.001924628236.67028836
GAACCGT601.7195052E-632.0774846
CGAGTCT350.004097523631.43167537
GCCGTAT801.5004844E-830.94055739
CGGGTAA805.1807365E-727.49885217
AACCGTG805.184975E-727.4962777
ACGGGTA957.974086E-826.05154416
GTATCAA66850.025.5205331
TTCGGGA550.001226461325.00012651
GGTATCA34550.024.0523971
ACACCGC600.002037593422.912496
AGAGTAC47350.022.41827811
TATCAAC77350.022.1190622
CAGAGTA48150.022.10291710
GTATAGA3000.022.0135151
CGAATTA2700.021.38699315
AGCCCCG2600.021.15395410
GCGAGCG650.003241265221.15197232
Produced by FastQC (version 0.11.5)