FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328368
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15120.4604589972226283No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13590.41386493202748137No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.34138527505725286No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10730.32676752911367735No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.2759099546849876No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8950.27256005457291815No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8400.25581055401257125No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.24454270818106516No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.20738927057447742No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5710.17389026945378355No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5660.1723675875846611No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.16323149636992643No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5070.1543999415290162No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.14313209569751012No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.13460507723042442No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4240.1291234225015836No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4240.1291234225015836No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.1288188861277591No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4170.12699166788481217No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.10110607610973055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA302.6428224E-670.838423
AAGTACG3500.061.9330141
ATACGGG1350.059.8191071
GTGGTAT7750.059.7784731
CGTTGTG302.252055E-456.670732
TGGTATC9100.056.515042
ATATCAA7000.055.861151
CTCGGTG354.8202166E-448.574912
GTGTAGC354.8202166E-448.574911
TCGGTGG354.8202166E-448.574913
GCGTTGT452.9208597E-547.225611
TAGCAGA409.313363E-442.4965744
AGTATCA2400.038.961131
GTAGCAG450.001660731437.7804873
GTATCAA30150.035.8068771
TATCAAC41350.035.7703972
AGGGCTC751.4812875E-533.997265
GAGCTAG250.002789222433.99725736-37
CGGTGGT500.002787201433.9972574
GGTATCA16050.033.631693
Produced by FastQC (version 0.11.5)