FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733554
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT71790.19228327754198815No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT71020.19022089944326503No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64470.17267729353854264No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.11506462743005727No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG203.0283272E-585.006421
TGGTATC38600.052.5212862
GTGGTAT41150.047.5127031
GTACGAC1056.91216E-1140.478163
GTATCAA144100.038.167351
GTCGTAC1053.0522642E-936.431321
GGTATCA75400.035.513291
TATCAAC173300.034.972382
ATACGGG6450.034.9251171
CAACGCA189550.031.8401175
GTCGCGC550.00444996630.908118
TCGCGCA550.00445025830.9076989
AAGTACG10750.030.8395371
ATCAACG198300.030.5852013
TCAACGC202300.029.9804524
AACGCAG205100.029.6126086
TAGTGCG755.906476E-428.3347135
GTCGAGG27000.026.758418
CGCAGAG226450.026.649638
ACGCAGA230050.026.3619127
Produced by FastQC (version 0.11.5)