FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998252
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66020.33038875977604426No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24100.1206054091275775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCT150.00578491154.99837529
GTGGTAT29450.043.086171
TGGTATC30700.041.386252
CGTCACT501.8222807E-532.9957218
AATATCG350.004105470631.4205635
ATACGCC400.00787371527.5005651
TGCGTAC507.034965E-427.49918725
GTATCAA115000.026.0646781
TACGATA651.08902044E-425.3857747
GGTATCA58550.024.5865211
AAGTTCG701.8004098E-423.5730955
AGAGTAC83700.023.09957911
CAGAGTA87050.022.27381110
TATCAAC134950.022.233322
TATACCG2150.021.7386465
CGTCTAA1155.043439E-721.52325840
GCACCGG650.003238781421.15586913
GCACGTC650.003239721821.1548141
GCGCACA1207.5965727E-720.62542350
CAACGCA144850.020.4418035
Produced by FastQC (version 0.11.5)