FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748387
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46000.263099645559021No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38370.21945942174129643No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.1500239935437635No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT23200.132693734281941No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT22420.12823247942246194No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT20480.11713653784888586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24800.051.7644271
TGGTATC25650.051.2032622
GTCGTAC709.238234E-636.435891
GTATCAA85250.034.9542351
GGTATCA45300.033.5001071
TATCAAC103700.032.953672
AAGTACG7300.032.6092871
GTCGAGG17700.031.4530248
ATCAACG111700.030.6696193
CAACGCA112350.030.4165155
TCAACGC112750.030.3086094
ATACGGG5150.029.7147081
TCGAGGT18500.028.9443119
AACGCAG119400.028.7985536
AGTATCA5350.028.6038761
AGTCGAG24300.027.4572947
ACGGGCG1956.91216E-1126.1561095
ATATCAA18800.025.0981271
CGCAGAG135800.025.0666988
ACGCAGA137300.025.0095127
Produced by FastQC (version 0.11.5)