FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819598
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37050.4520508834819021No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25860.31552053567724664No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.272328629401243No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT21590.26342182386975077No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19270.2351152638244603No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.23377314244300254No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18340.22376823759940848No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.20839484722998347No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA12650.15434395886764No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11940.14568117540550365No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA11400.13909257953289295No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA11020.13445616021512985No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10790.1316499064175364No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10570.12896566365462092No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.12652544296106139No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.11334825121583995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG1850.073.533781
TGGTATC23900.060.825242
GTGGTAT22100.057.7082181
TAGTTAG250.007790725751.0078355
GCCTACG354.8196188E-448.584821
AGTATCA3950.046.2785841
AGATACG409.3107985E-442.506536
GGTATCA41650.036.2300573
GTATCAA78050.035.3492741
ATATCAA16450.034.8880351
CTAGCGA751.4816083E-534.0052226
CGGACGT250.002788899534.00107638-39
TATCAAC99300.033.3888172
TAGCGAG802.3058981E-531.8760077
TAGAGCG802.3078228E-531.8721189
ATGGTAT6100.031.3611031
GTACCGC550.004443929530.9138376
GATACGG550.004446584330.9100697
AAGTACG6350.030.7958891
GTCGAGG18450.030.1752628
Produced by FastQC (version 0.11.5)