FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777752
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.19890659233277444No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14890.19144920231641963No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13940.17923451177239016No Hit
ACATATAACAGTGCATGCATACATTCAAGTTATATATCTAACATGGAAAATTGGTAAATTT11550.1485049218774108No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.11250372869500817No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.11173227455538526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24500.050.763491
TGGTATC25650.048.4625932
TCGCGTC401.8989574E-434.38123339
GTCCTAG350.00408745831.4464071
GTCTACT453.7818291E-430.5728991
CGGGACG553.4896842E-529.9996517
CGGGATA705.704336E-627.49967817
GGTATCA54450.026.7323361
CAGTGGT1800.025.9869631
AGTGGTA1800.025.973592
GTATCAA105900.025.9306641
CGGGGCA1750.025.14256317
CAGAGTA71750.025.06752210
CCGGCGT550.001224902225.00453239
CGCGTCA550.001224902225.00453240
AGAGTAC73750.024.38772411
TATGACT1052.107372E-723.5590294
CTACACT952.3249904E-623.1457144
ACGGGGC3350.022.98480616
CGGGGGT3000.022.91639717
Produced by FastQC (version 0.11.5)