FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93783
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT46744.983845686318416No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.9202094196176279No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT8050.8583645223547978RNA PCR Primer, Index 47 (95% over 22bp)
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.8540993570263268No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA7040.7506690978109039No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.6440399645991278No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT5700.6077860593071239RNA PCR Primer, Index 47 (95% over 24bp)
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA3990.4254502415149867No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA3940.4201187848543979No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.3742682575733342No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.3454783916061546No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA3130.33374918695285927No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.3262851476280349No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.31242336031050405No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT2600.27723574635061793No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA2580.2751031636863824No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA2530.26977170702579356No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA2420.2580425023724982No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT2300.24524700638708508No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC2290.24418071505496733No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.2335178017337897No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.2057942270987279No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC1800.19193243978119703No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.1727391958030773No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.17167290447095956No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCA1580.16847403047460627No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.16314257381401745No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG1450.1546122431570754No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC1430.15247966049283984No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC1370.14608191250013328No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA1330.14181674717166226No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGCTCTCGTATGCCGT1300.13861787317530896No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1280.13648529051107344No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATGTCGTATGCCGT1280.13648529051107344No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT1210.1290212511862491RNA PCR Primer, Index 47 (95% over 21bp)
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.12582237718989583No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.12368979452566031No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA1160.12368979452566031No Hit
GAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.11942462919718926No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.11729204653295373No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT1080.11515946386871821No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.11196058987236492No Hit
TATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAAC1050.11196058987236492No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA1050.11196058987236492No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.11089429854024715No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCG1010.10769542454389389No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.10662913321177613No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA990.10556284187965836No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.10129767655118732No Hit
GCCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC940.10023138521906955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG800.074.3751
GCGTCAG302.6292219E-670.833331
ATATCAA1450.067.413791
TGGTATC4900.053.7755132
GTGGTAT4850.053.453611
ATATAAG250.0077740750.9999961
GGAGGAC250.0077740750.9999963
CGCGAGA2500.049.32
CCGCGAG2550.048.3333361
CGTCAGA452.90602E-547.222222
GCGAGAT2700.045.6481483
CGAGATG2800.044.0178574
GTCGAGG3750.039.6666688
TCGAGGT3550.039.5070429
GTCAGAT559.4948235E-538.636363
TCAGATG559.4948235E-538.636364
ATACGGG805.3423173E-737.18751
GAGATGG3350.036.7910425
AGCGCCG858.58714E-735.02
GTATCAA16050.034.6884771
Produced by FastQC (version 0.11.5)