FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055764
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52750.25659560144063226No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT50850.24735329541717824No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.2051305500047671No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.1434503182271895No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT27750.1349863116583421No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.10974022309953865No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20820.10127621653069127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24700.058.8527761
TGGTATC28000.055.1030772
AAGTACG8550.038.776291
GTATCAA100000.034.8114131
GGTATCA53950.034.0353361
TATCAAC121750.033.4444272
ATACGGG5050.031.9837951
ATATCAA20250.030.435521
ATCAACG134350.030.1503933
CAACGCA134900.029.8699285
TCAACGC136750.029.5590844
AGCGTCA1751.8189894E-1129.1461373
AACGCAG144450.028.0716936
GTCGAGG23500.027.8514868
AGTCGAG30400.025.1660797
TCGAGGT24600.024.7044569
CGCAGAG168300.024.0155168
ACGCAGA169350.023.943077
CCGTAGG900.001710402923.6137661
GCAGAGT174900.023.1567469
Produced by FastQC (version 0.11.5)