FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211268
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.3038794327583922No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.2679061665751557No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT3750.17749966866728517No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.16803301967169662No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.1590397031258875No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.13205975348846016No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.12259310449287161No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.12211977204309218No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.11265312304750365No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT2180.1031864740519151No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.10034647935323854No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.10034647935323854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG401.9496656E-763.7475171
GTGGTAT2400.054.89371
TGGTATC2900.052.7565652
GGGATCT409.3038986E-442.4983445
GTATCAA12700.040.825181
TATCAAC15750.040.7444422
TCGAGGT3450.040.650599
GGTATCA6100.038.318181
GTCGAGG3600.037.7763068
ATCAACG17950.035.513943
ATATCAA3500.035.2129141
CAACGCA18000.035.1791885
TCAACGC18300.034.834714
TAGACAC500.002784393733.9986765
TTATCCC500.002784393733.9986764
AGTCGAG4700.033.4561427
AACGCAG19800.032.1957136
GATCGTC300.00680207928.33223226-27
ACGTTCC300.00680207928.33223246-47
CAGGTAT905.1368705E-528.3322281
Produced by FastQC (version 0.11.5)