FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211268
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14540.688225381979287No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4350.2058996156540508No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.13584641308669557No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA2210.10460647140125337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG150.005774738355.00568413
GTGGTAT3750.044.0358281
TGGTATC4350.039.1994552
TGCTTAA458.803787E-636.67045619
GCCCCGA501.8117218E-533.0034111
GTTCTCA350.00409005931.4318189
ATAGGAC350.00409005931.4318183
ATACCAC653.186833E-629.61844412
GTATCAA16400.028.8649481
CCGAATT606.28933E-527.50284214
AGGACAG507.0279976E-427.4898225
TATCAAC17750.026.8055882
ACCACTG759.695554E-625.66931714
TAATACC651.08408596E-425.3872420
CGAATTA651.08408596E-425.3872415
CTACAGA651.0875814E-425.3752214
TACAGAG651.0875814E-425.3752215
GTATTCT550.00121744725.0203591
ATGCACG550.001224193424.99666424
GATTGTC550.001225884824.9907477
Produced by FastQC (version 0.11.5)