FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309622
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.3333096485391865No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.2909999935405107No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.17989677736078186No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.13758712236210605No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.1366181989651898No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.13145060751496987No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT3820.12337624587400119No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.11045726724845134No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.10076803327928893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4150.051.2063752
GTGGTAT3950.049.5031741
GTATCAA17400.048.6156541
TATCAAC20050.047.2707632
AAGTACG1550.046.6218531
ATACGGG952.3646862E-1144.745431
AGTATCA1200.042.508161
CAACGCA23550.040.064915
ATCAACG24300.038.8283423
TCAACGC24850.037.9689644
AACGCAG25550.037.4277536
ATACCTT709.224472E-636.4296766
CGGGAAC1254.4201443E-1034.0010346
GGTATCA6700.033.625861
ACGGGAT652.5486134E-432.6933023
CGCAGAG29000.032.3836868
ACGCAGA29800.031.9472797
GCAGAGT30250.031.1859959
ATAGGAC755.894775E-428.3341943
AGTGTCC600.00680237428.3296188