FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314780
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17690.5619797954126692No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6440.20458733083423344No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4870.15471122688862063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAC150.00577628255.0082724
GTGCGCT150.00577628255.0082721
TGGTATC4250.038.8170242
GTGGTAT4500.036.0552441
TTAAACG558.663028E-735.0052622
AAGCTAG350.004088950331.43829744
TCGGTGC350.004098510431.42330418
GTATCAA19850.028.2617191
GCGTGCC400.00786491827.49976212
CTGAACC400.00787102227.4953923
TATCAAC22200.025.2660312
GGTATCA9200.024.5106561
CGAGGGT1151.8992068E-823.91283610
AGAGTAC13750.023.79979311
ATCAACG23300.023.6011923
TCAACGC23450.023.3292684
CAGAGTA14400.023.10743710
CAACGCA24050.022.9722125
CCTAATA600.00203424622.9128272
GATACCA1003.6017518E-621.99980711
Produced by FastQC (version 0.11.5)