FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61797
Sequences flagged as poor quality0
Sequence length61
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31935.166917487903944No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9781.5826010971406381No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.5226790944544234No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.35114973218764667No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC2050.33173131381782284No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.24111202809197857No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA1350.2184572066605175No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA1340.21683900512969884No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.20551159441396832No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC1090.17638396685923266No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA860.13916533165040373No Hit
GAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.13754713011958508No Hit
GTGGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.13269252552712915No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAAT780.12621971940385454No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.12298331634221725No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.11812871174976132No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCGTACTCTGCGTTGATAC670.10841950256484942No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT202.59444E-454.9991917
ACTTTCC150.005758156554.999198
CTTAAAC202.59444E-454.9991921
CACTGCT257.7992445E-443.9993516
TGCTTAA257.7992445E-443.9993519
CTGCTTA257.7992445E-443.9993518
GCTTAAA257.7992445E-443.9993520
TAAACGC300.001911702136.6661323
CGGGAGA300.001911702136.6661317
GTTGCTG300.001911702136.6661341
TTAAACG300.001911702136.6661322
TTGCTGT300.001911702136.6661342
GTGGTAT3750.035.2280041
GGGGAGT401.8810084E-434.37449318
TGGTATC3950.033.417232
ACGGGAG601.682849E-632.08286316
GAGCGCC553.4195367E-530.023871
AGCGCCG553.4385434E-529.999562
CGGGGGA752.8699833E-729.33290317
ACGGGGT805.0477684E-727.49959616
Produced by FastQC (version 0.11.5)