FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24243
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT5612.31407004083653No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.7094831497751929No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.5857360887678918No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.5733613826671616No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.4784886358948975No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.4784886358948975No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC1120.4619890277605907No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.4207400074248237No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.346491770820443No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.2763684362496391No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.263993730148909No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.25574392608175556No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.24336921998102545No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.22686961184671864No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG530.21861980777956522No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.21861980777956522No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT520.21449490574598856No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT500.20624510167883514No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT490.20212019964525843No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGGCGT470.193870395578105No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.193870395578105No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGG470.193870395578105No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT440.1814956894773749TruSeq Adapter, Index 7 (95% over 21bp)
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.1691209833766448No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.1691209833766448No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.1691209833766448No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.14024666914160788No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC340.14024666914160788No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA330.13612176710803117No Hit
ATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.12787196304087778No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.11962215897372437No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATGTCGTATGCCGT280.11549725694014767No Hit
ATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11549725694014767No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA260.10724745287299428No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTTTGCCGT250.10312255083941757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG258.5893043E-785.173911
GTGGTAT600.078.076081
TGGTATC700.072.855372
ATATCAA1050.060.838511
GTATCAA2950.047.6396451
GGTATCA1250.047.5988463
TATCAAC4300.047.4407122
GGCGTCG209.2321454E-442.49896646-47
ATCAACG4900.041.631643
TCAACGC4950.041.2111174
CAACGCA5200.039.2298165
AACGCAG5600.037.1865926
TCGTTTG250.00276313233.99917240-41
TGGCGTC250.00276313233.99917244-45
CGCAGAG6400.032.5382738
ACGCAGA6500.032.037687
GCAGAGT6500.032.037689
TGCGTGA408.49352E-426.56185354-55
GTCGACG808.3704054E-426.5618538
AGAGTAC3750.025.4993810-11
Produced by FastQC (version 0.11.5)