FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24243
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18167.490822092975293No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1890.7796064843459968No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.7507321701109598No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.39186569318978676No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.3794909870890566No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.24749412201460216No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.1608711793094914No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.13612176710803117No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1319968650744545No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.12374706100730108No Hit
GTGGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11549725694014767No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT270.11137235490657096No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.11137235490657096No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG150.005716528754.9979325
GGGCTCG150.005716528754.9979324
TGGTATC900.048.887052
GTGGTAT800.048.2228281
CGGGGGA401.8476677E-434.37370717
GTATCAA4100.027.5559021
GGTATCA1850.026.8111461
TATCAAC4500.025.0546132
CAGAGTA3000.022.91580810
GGGGGAA600.00197929922.91580618
ACGGGGG600.00197929922.91580616
ATCAACG5050.022.3258953
AGAGTAC3100.022.17658411
TCAACGC5100.022.1070144
CAACGCA5200.021.6818775
AACGCAG5400.021.3880866
GACTTTT1008.53656E-519.2492774
GAGTACG3550.018.5908512
AGTACGG3450.018.33264513
CGACTTT1051.2414188E-418.3326443
Produced by FastQC (version 0.11.5)