FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146247
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8630.590097574651104No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4270.2919718011309634No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.17641387515641346No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.1360711672718073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3650.038.4888231
TGGTATC4100.034.9123652
ACAAAGG400.00785271327.498849
CAGGGTG400.00786583927.4893997
GTATCAA14800.025.8708321
TAGTCCT651.08293E-425.383514
GTCTGAT550.001209604825.0417461
TATCAAC16150.024.3737982
TTACTCC701.7907028E-423.57040240
GGTATCA7750.023.4584621
AGAGTAC9550.023.32359111
CAGAGTA9650.023.08189610
TACAGAG600.002027605422.9156685
ATCAACG17250.022.3255063
TCAACGC17450.022.2196644
CAACGCA17850.021.7217435
GTAGTCC650.003220819621.1601643
CAATCGA650.003227221821.15292525
AACGCAG18900.020.5079656
AGCTTCA700.004956919719.64200254
Produced by FastQC (version 0.11.5)