FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32451
Sequences flagged as poor quality0
Sequence length91
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5331.6424763489568888No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4201.2942590367014883No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA4191.2911774675664849No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.8967366182860312No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA2050.6317216726757265No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.628640103540723No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA1950.6009059813256911No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA1740.5361930294906166No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.5084589072755847No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA1640.5053773381405812No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT1630.5022957690055776No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.46531693938553514No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.43450124803549967No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT1370.4221749714954855No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.317401620905365No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.3112384826353579No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.30815691350035435No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.30815691350035435No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.2773412221503189No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.27117808388031184No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.27117808388031184No Hit
AGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA770.23728082339527287No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.21879140858525162No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.20646513204523745No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.1972204246402268No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.1910572863702197No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.18797571723521617No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.18181257896520908No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG550.1694863024251949No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.16640473329019137No Hit
ACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.16024159502018429No Hit
AAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.15099688761517363No Hit
ATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.14791531848017012No Hit
GAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.13250747280515238No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA430.13250747280515238No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG390.12018119626513821No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.12018119626513821No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.11709962713013468No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA380.11709962713013468No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA370.11401805799513111No Hit
GTGGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAA360.11093648886012758No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.11093648886012758No Hit
CAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAA330.10169178145511694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)