FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211903
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20720.9778058828803745No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5270.2486986970453462No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5010.2364289321057276No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT2940.13874272662491802No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT2700.12741678975757778No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.12741678975757778No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGAT2570.12128190728776846No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT2510.11845042307093341No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.11514702481795916No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.11467511078181998No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC2250.1061806581313148No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTC2220.10476491602289728No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTGAACAGGAG2130.10051768969764467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3700.042.359052
GTGGTAT3950.040.393271
ACGGGAT504.1077874E-738.49471316
CGAGGGT1150.035.8646410
TCGAGGG1150.035.864649
CCGAATT350.004094909431.42425514
GGACCGA350.00409964431.4168426
GTATCAA17100.028.6353151
AGAGTAC12600.028.15089611
TCCTACT507.018505E-427.4962252
TGGACCG400.00787322227.4897335
CAGAGTA12950.027.3900610
TATCAAC18400.026.898482
AGGGTTT1650.024.99656712
GAGGGTT1650.024.99656711
AGTACGG14000.024.353813
GGTATCA7700.024.2938461
ATCAACG20550.024.0786023
TCAACGC20500.023.735044
CAACGCA20850.023.2047655
Produced by FastQC (version 0.11.5)