FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380147
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.37669638324121985No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.2875203539683333No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10840.28515284876639824No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7640.2009748860309301No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.19045264068899664No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7130.18755902321996493No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.18440234961738486No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT6980.18361318121673983No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.1630948027999695No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.154940062659971No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT5250.13810447011287738No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.10995746382320523No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.10469634115223847No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.10180272368320675No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG200.00322182363.753
CCGCGAG200.00322182363.759
TGGTATC8200.059.0853652
ATACGGG1300.058.8461531
GTGGTAT7600.058.1578941
AGTATCA2150.051.395351
AAGTACG3950.049.493671
ATATCAA7500.044.21
TCAGCGA209.317416E-442.530-31
TATCAAC41350.038.5429272
GTATCAA32650.038.2695241
GGTATCA16450.037.4620061
ATGGTAT1301.6370905E-1135.9615361
TGACGAT250.002788395434.024-25
ATCAACG47950.033.1491133
CAACGCA48350.032.874875
AGCGTCA652.5501565E-432.6923073
CTAGTGG652.5501565E-432.6923073
TCAACGC48950.032.4719124
AACGCAG52300.030.473236
Produced by FastQC (version 0.11.5)