FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114711
Sequences flagged as poor quality0
Sequence length91
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11601.0112369345572787No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA9610.8377574949220215No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.7636582367863588No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT7930.6913024906068294No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT6370.5553085580284367No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.5239253428180384No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA5070.4419802808797761No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.3835726303493126No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.37136804665637996No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA4250.37049629067831336No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.33126727166531544No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.32254971188464926No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA3700.32254971188464926No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA3690.3216779559065826No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.3190626879723828No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3060.2667573292883856No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.24845045374898658No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.2231695303850546No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.20922143473598873No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.1952733390869228No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.19004280321852307No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.17086417170105744No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.15691607605199154No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.14907027224939196No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.1386092005125925No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.13686568855645928No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.12117408095126013No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.11507178910479378No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.11420003312672716No Hit
AGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA1290.11245652117059393No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTATGCCGT1270.11071300921446069No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.10984125323639407No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.10722598530219421No Hit
GAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.1028672054118611No Hit
AAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.1028672054118611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG1050.076.91661
ATATCAA2700.070.844241
CAGTATC302.2449408E-456.6753851
CCTTATA250.00776033551.0301062
TAACAGT250.00776033551.0301064
ATACGGG751.10958354E-1051.007851
AGTATCA1400.048.5789071
CTGCTTA255.4333417E-542.50654218-19
TGGTATC6100.039.7365572
TATCAAC28450.037.966162
GTATCAA23100.037.9062651
GTGCGTG301.5941991E-435.40667754-55
GTCGAGG4950.035.2074368
ATGGTAT1600.034.5365641
GTGGTAT5300.034.486441
CCGGGGT250.002776309634.02007346-47
GACATTC250.002776309634.02007330-31
TTGTGCT250.002788211233.9904152-53
AACGCAG31950.033.6740156
ATCAACG32100.033.3841823
Produced by FastQC (version 0.11.5)