FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114711
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46104.018795058887116No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13021.1350262834427387No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4500.3922901901299788No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.38008560643704614No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160.27547488906905176No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.23798938201218717No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.20834967875792207No Hit
GAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.18917104724045644No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.15342905213972505No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.1333786646441928No Hit
GTGGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.1246611048635266No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.10199544943379449No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAAAA150.00576656355.0091725
ACCTGCC150.00576656355.0091738
ATCGTCC150.00576656355.0091717
TGCTTAA257.820362E-444.00733619
CTGCTTA300.001916830336.6727818
CTTAAAC300.001916830336.6727821
TTAAACG300.001916830336.6727822
CACTTTA300.001916830336.6727814
CTCCCAG300.001925070336.640781
ACTGCTT401.8890521E-434.38073317
GTATAAG757.930794E-932.9767041
ACGGGGT1400.031.43381316
AGAGACA701.6035301E-731.3926896
TGGTATC6400.030.043782
CGGGCAA653.1685013E-629.62032117
CGGGGTG952.4410838E-928.95219617
GTGGTAT6550.028.949011
ACGGGCA1350.028.52327316
GTATCAA28000.028.363891
AGAGTAC17900.028.27287111
Produced by FastQC (version 0.11.5)