FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058391
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111110.5397905451393832No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52320.2541791136863696No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29880.14516192501813308No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27530.13374524082159317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCTA150.005784795654.99870711
GTGGTAT47700.042.6245421
TGGTATC48800.041.530792
CTCGTCG358.6675806E-539.28766336
ACGACTA358.672547E-539.2838427
CGGACCC300.001926198136.66580622
TACATCG658.3900886E-833.850341
CGCGCTC350.00410036231.42860233
ACTTCGG350.0041008531.42783515
CGAACGG350.00410182631.4263118
TACGGCC350.004106219431.4194344
CACGTCG453.7965595E-430.55558229
TCGCAGA453.7981823E-430.55335617
CGTACAC553.4938883E-529.99783913
ATAGACC653.2079042E-629.614693
GACGCCC400.00787171927.5020349
CGTCGGG400.00787265227.50136238
CTGTCGG400.00787545527.4993559
GTCCGCG400.00787732327.4980213
ACCGAGC759.748055E-625.66981140
Produced by FastQC (version 0.11.5)