FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371349
Sequences flagged as poor quality0
Sequence length91
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41831.1264336244341577No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40281.084693913272959No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28280.7615477623475491No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA25850.6961106667851537No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT25470.6858777053391824No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.6072454752806659No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA18150.4887585532746823No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17950.4833727840925921No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.4190128423666147No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.4044712655749712No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA13310.35842293906810035No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA11170.3007952088197356No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.29460157426033196No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.2908315358328688No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.28975438199645076No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.28194501668242006No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA9740.26228695916779093No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA9690.26094051687226844No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8210.2210858249248012No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.20654424813315778No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.19200267134151433No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.17988469068181145No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6160.16588169080837703No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGT6100.16426596005375No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT5980.1610344985444959No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.15941876778986883No Hit
CTCCTGGGATGTGAATAAAGAAAATACGTCTGAAAGGTGAGGATTTCAAA5370.14460790253912087No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT5340.14380003716180736No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.14056857565255326No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.13625996030688112No Hit
AGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA4960.13356707571583604No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA4810.12952774882926843No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4770.1284505949928504No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.12683486423822335No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4650.12521913348359628No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4410.1187562104650881No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC4130.11121613361016187No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC4120.11094684515105736No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCA4110.11067755669195287No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.10933111439643031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAA7650.073.3360061
AAGTACG2850.073.072841
GTCTAAT302.6440575E-670.835911
ATACGGG1350.066.1135251
TACTGGT200.003221298663.752322
CGTCGAG200.003221298663.752321
CGATGAA200.003223018363.7437369
AGTATCA3550.057.466881
GCAATAC302.2528334E-456.6687281
AATACTC401.4560113E-553.1269345
ACCGTCC651.7953425E-952.3025558
CTATCCT250.00779099651.001864
AGGTACG250.007795145750.9949959
TCTAATA354.8218752E-448.5731962
CTCACTA354.8250795E-448.5666549
GTGGTAT19450.047.199661
TGGTATC21150.046.2191772
TGTATAA4600.044.349442
AGCGTCA505.4436437E-542.501553
GTGTATA4400.042.501551
Produced by FastQC (version 0.11.5)