FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071081
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT91170.4402048978287184No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78350.3783048562562256No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT54640.26382357812176344No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52410.2530562541976871No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.20119927709249422No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38270.18478272940556165No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30630.14789378107374843No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28650.1383335562442995No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC28640.13828527228051438No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28470.13746444489616774No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA28450.13736787696859756No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC27890.13466397499663219No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27180.13123581356789038No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA25640.12380008314498564No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA23530.11361216678633043No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT23020.11114968463329054No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACG22650.10936317797324199No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT22190.1071421156391276No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA22130.10685241185641702No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT21660.10458306555851751No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21650.10453478159473241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC46050.054.543172
GTGGTAT45650.052.046971
AAGTACG10200.045.003681
ATATCAA31000.041.8179361
AGCGTCA1550.038.386533
CGTATCA450.001662686137.780871
AGTATCA14150.036.646111
GTCGCAA1053.0504452E-936.4315531
GCGTCAG1650.036.0600744
GTATCAA159450.034.9197581
GGTATCA84900.034.7437131
TATCAAC201050.033.9487652
CTAACGC1850.032.161693
TCTGTCG1201.12413545E-831.8745278
ATAGGCG952.0757107E-631.3153276
ATCAACG225000.030.335113
CAACGCA228950.029.8303095
TCAACGC230950.029.5719834
TCGAGGT42050.028.7034999
AACGCAG242300.028.3972286
Produced by FastQC (version 0.11.5)