FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071081
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109590.5291439591208649No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37040.17884380185999485No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27060.13065640600246922No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT26650.1286767634872803No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT26550.12819392384942935No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC26480.1278559361029337No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT23960.11568837722908953No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGAT23880.11530210551880878No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGG22600.10912175815431652No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACGTTAAAAACTTA21860.10554874483421943No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA21850.10550046087043433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC150.005787099254.9931874
GTGGTAT50500.040.9305231
TGGTATC51950.039.2336272
AGTCGTT752.9477997E-729.33253521
AACGGTT400.00787190127.50190753
ATGTCCG400.00787375627.50058215
GAACGAT1500.025.66596816
AGCCGTC651.0892198E-425.3851538
CGGAAGT550.001226217725.00233812
CGCGGGC550.001226735925.00052845
TAGATCG1551.8189894E-1224.84103410
GTATCAA187700.024.6491581
GGTATCA101000.024.0896021
GTCGTAG952.3170523E-623.15838238
GTATCGG1553.6379788E-1123.0839611
AGAGTAC127650.022.96728911
GTCGCAA600.002026125322.9359871
ACCGCGG600.00203582822.9171543
CGAGTCG600.002037258122.91438128
CAGAGTA129450.022.71166410
Produced by FastQC (version 0.11.5)