FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314484
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT38221.21532415003625No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31821.011816181427354No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.9374085804047265No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA25730.8181656300479515No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.6728482212131619No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15600.4960506734841836No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA14990.4766538202261483No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.38666514035690214No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11760.3739458923188461No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA11260.35804683227127615No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.3459635466351229No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA10390.33038246778850433No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGT9740.30971368972666335No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA9000.28618308085625976No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA8060.2562928479668282No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.2483433179430432No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.24134773152211242No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.21972500985741722No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.21877106625456302No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.18315717174800625No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT5580.17743351013088107No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT5010.15930858167665127No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT4720.1500871268490607No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.1500871268490607No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.1484972208443037No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4310.1370498976100533No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT4280.1360959540071991RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.13482402920339348No Hit
AGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA4070.1294183487872197No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGG3920.1246486307729487No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.12433064957199731No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.1205148751605805No Hit
GTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGG3760.11956093155772632No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC3690.11733506315106651No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.11669910074916372No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA3510.11161140153394132No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3430.10906755192633011No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC3350.10652370231871892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG2200.079.2035141
ATATCAA4650.063.9776571
ATACGGG1450.058.619921
AGTATCA2400.051.3534971
GTGTTGC250.00779142150.9993361
TAGGCCC250.00779142150.9993364
AGTACAT250.00779142150.9993368
GTGGTAT14950.047.758571
TGGTATC16050.046.603752
ATATAAG1250.044.199421
TATAGTG603.2016796E-642.4994472
ATAGGCC450.001661317137.777283
TACCGTG450.001661317137.777287
GTGTATA5150.036.3102041
TATACAG601.5930212E-435.4162064
TGTATAA5550.035.2247622
GTATCAA60150.035.1159171
CAAGTAC500.002786100133.9995573
CCGTGAG500.002786100133.9995579
GTCAGAT1501.8189894E-1233.9995542
Produced by FastQC (version 0.11.5)