FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314216
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT99700.300825293221685No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88780.2678763242950972No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60810.18348230773130056No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT55430.16724920765574725No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50540.15249458695510493No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.12669663051533153No Hit
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTC37490.11311875870492448No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37010.11167045237848107No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35140.10602809231504524No Hit
ATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAGGCATACTCATC34280.10343321014683413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC53650.056.0134432
GTGGTAT52000.055.754841
ATACGGG6550.055.1669771
AAGTACG17950.039.787481
GTATCAA187750.034.1446761
TGCTACG250.00279031533.9990718-19
TATCAAC242000.033.2138142
GTCGAGG45450.032.44498
GGTATCA107900.031.8734191
AGACGTT1509.276846E-1131.1695776
GTACTAG4150.030.7308621
ATCAACG263650.030.5020773
TCGAGGT46000.030.2093099
CAACGCA268000.029.8796565
CTAACCA1452.1864253E-929.3130824
TCAACGC274800.029.2949494
AGTATCA17300.029.241691
AGTCGAG59500.029.2130457
TCGGTAC300.00680879928.33255840-41
AACGCAG293150.027.5916986
Produced by FastQC (version 0.11.5)