FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314216
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172410.5202135286293953No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51140.1543049698631592No Hit
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTCATCCAATCCAA41030.1238000178624447No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35550.10726518730221567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC56150.040.6561472
GTGGTAT57150.040.4461671
CGCGTCC358.674386E-539.2833122
ACGTTCG704.087269E-935.3587144
GCGCGTC401.9030007E-434.37289421
CGAGTCC2150.031.97430627
GTTCGTT852.7112037E-829.1189446
TTCGTTA852.7121132E-829.11806347
TTCGTAA1052.3101165E-1028.80993351
CCTAGCG400.00786947127.504132
GACGCGC400.00787526927.49997910
ACAACGC1103.965397E-1027.4983184
CGTCCCG507.037872E-427.49790224
CGCGACT805.1905954E-727.49624317
AGACGTT1203.6379788E-1127.4954136
GACGTTC957.9780875E-826.0537943
GTACTAG2750.026.0168651
GTATCAA219050.025.237771
CGAGTCA550.001226940225.00035751
CATAGCG1650.024.9969625
Produced by FastQC (version 0.11.5)