FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4120468
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT92000.22327560849884043No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81360.19745329899419195No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.1310773436415475No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT50270.12200070477431203No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50060.12149105392882556No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT41660.10110502010936623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT46500.051.921221
TGGTATC58750.043.335332
ATACGGG13800.038.5017471
GTATCAA183650.035.898011
AAGTACG19350.034.0486761
TATCAAC231350.033.859382
ATCAACG246950.031.6688273
CAACGCA252350.030.9069335
TCAACGC253300.030.8413564
GGTATCA104500.029.0830021
ATATCAA45150.028.9021471
AACGCAG274800.028.6449096
AGTCGAG60600.026.9295487
CTAGACG1308.031202E-726.1559074
GTCGAGG52700.025.8050428
TCGAGGT51500.025.6630219
CGCAGAG311500.025.0620338
GCAGAGT321700.024.3592829
ACGCAGA326100.024.0445167
AGTATCA17650.022.8785461
Produced by FastQC (version 0.11.5)