FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233330
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT23380.10468672341302002No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12550.044.7101171
TGGTATC14400.038.3705642
GCGTCAG1301.6370905E-1135.963894
GCATCGT3850.035.322248
TAGTACG1254.456524E-1034.002224
GTACTAG5700.032.8135721
GGTATCA31750.030.2580091
GTATCAA54050.029.6497691
TATCAAC59850.029.0453412
TCGTAGA600.006804765728.3351862
CTAGTAC7700.028.151193
CAACGCA59150.028.0956655
ATCAACG64300.026.5724983
GACTGCG1159.182801E-625.8683597
TCAACGC67000.025.4382294
CTGCGGA2201.0913936E-1125.1119029
GTCGAGG7450.025.0989388
ACTGCGG850.001227410624.9982768
TACTAGG8050.024.2873022
AACGCAG69400.024.2523026
Produced by FastQC (version 0.11.5)