FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233330
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62270.2788213116735995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12000.037.6017271
TGTTACG558.702009E-735.0014448
TCGTCTA3550.034.08514854
CGGGGTC658.403367E-833.84451317
CTGGTCG3750.032.99987850
CAATGCG2400.032.0817819
CACGGGC350.004101427731.42704831
TGGTATC13700.031.1158122
ACGAGTC2300.031.0875426
AGCGCTC453.7952687E-430.55749739
GCGACCG453.7962652E-430.55612826
TATCGAT453.7962652E-430.55612841
CGCCAGC4100.030.18416834
CGCTGGT4200.030.12028748
GCTCGTC4300.030.0600539
GTCGTCA4300.030.05870224
TGGTCGT4050.029.87710451
GTCAGCG4450.029.66480643
CGTCGTA653.207011E-629.6159452
GTCGTCT4250.028.4711253
Produced by FastQC (version 0.11.5)