FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3536496
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT69880.19759671720256436No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59610.16855667304586233No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.15060104691197163No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA40800.11536843248232148No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT40420.11429392257194693No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.11358700815722682No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA38750.10957173428161661No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC38390.10855377752441965No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC37420.10581094959530564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT35350.052.1854481
TGGTATC40750.048.1866342
GGTATCA74750.034.8008081
GTATCAA121100.032.362041
AAGTACG8400.030.8673731
TATCAAC160150.028.8214052
ATACGGG6500.028.7732181
GTCGAGG30800.028.6999998
TCGAGGT30900.027.5072359
ATCAACG169550.027.223523
CAACGCA171050.026.8853955
TCAACGC176200.026.1960724
ATTAGCG1001.0567509E-425.4992077
AACGCAG183300.025.227766
AGTCGAG40650.024.6732797
TAACGCC450.001712032723.61087676-77
AGTATCA15250.023.413081
AGAGTAC100750.022.50505810-11
CGGCTAG950.002344485122.3677259
CGCAGAG206150.022.2849488
Produced by FastQC (version 0.11.5)