FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589626
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.10987217052695741No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT39320.10953787386206808No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA37020.10313052111835606No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA36590.10193262473583597No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36370.10131974751687223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.037.95041
GCGGTAT450.001662321437.7833061
GTGGTAT26700.036.9342461
TAGAGCG652.5532785E-432.6948134
GTATCAA82350.032.518421
TGGTATC32300.031.7138442
ACGGGTA2100.028.3355065
TATCAAC108650.028.0885332
CAACGCA107500.027.9533085
AACGCAG113100.026.4564936
TCAACGC118850.025.7487134
ATCAACG120600.025.376143
AAGTACG8850.024.9754071
AGTCGAG23100.023.7342647
AGGCGTC900.00171225523.6099629
GTCGAGG18550.023.3687868
ACGCAGA133350.022.4057947
TCGAGGT19200.022.3556849
CGCAGAG134700.022.148768
AGAGTAC75100.020.99431810-11
Produced by FastQC (version 0.11.5)