FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500580
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87691.7517679491789524No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15260.3048463782012865No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTC9930.19836989092652524No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT9700.19377522074393705No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT9540.1905789284430061No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9310.18598425826041792No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGAT8890.17759399097047426No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAAC8500.1698030284869551No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC8150.16281113907866876No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7850.15681809101442326No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGAT7810.15601901793919054No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGT6370.12725238723081228No Hit
GTAATGAGGCGTGCGCCGCCAATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTT6170.12325702185464861No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTC5940.11866235167206042No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCA5620.11226976707019856No Hit
GCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGT5530.11047185265092493No Hit
TAGATAGACAGATGGGAAAGGCAGGCGCGGCCTTCGTTGTCAGTAGTTCTTTGATGTGAAA5400.10787486515641855No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT5340.10667625554356946No Hit
CATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAGTTAAACAGCTAAAAGAAGTAAAA5270.10527787766191217No Hit
GGGCAGCACAGTCATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAGTTAAACAGCTA5260.105078109393104No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTGAACAGGAG5070.10128251228574853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT358.6577355E-539.28834
GTGGTAT17200.039.0529251
TGGTATC17250.038.5973852
GTGACTA300.001923092136.67246238
ACGAACT300.001924980536.66513446
GAGTCAG601.7178863E-632.075587
TGATAGC350.004094272431.4335436
TGAATCA350.004100285531.42411626
GATACTA453.7956634E-430.55122620
AGTCTCT1400.029.46305550
AGCACAT752.9431976E-729.32917617
GGACTAT1700.029.113526
GTATAAT400.00782842927.5291121
TATTAAG705.67823E-627.5125982
TCTAAAG400.0078552227.5098483
CGATTTG400.00787056127.49885231
GACGAAC400.00787056127.49885245
GTGCGCT400.00787056127.49885211
GCCCGAC705.7003435E-627.49885242
ATCTAGC400.007874427.49610326
Produced by FastQC (version 0.11.5)