FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5609325
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180970.32262348856591483No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTTGCTTTTCCTCCGCAACCATGTCTGAC63650.1134717635366109No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT60970.10869400507191151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA709.458745E-1139.28715543
TGGTATC36550.035.0653462
GTGGTAT38250.034.5310861
TTAACGG1101.2732926E-1130.00163735
GGTATCA74400.027.0731351
TATAGCG1251.7498678E-924.19894628
GTATCAA137500.022.053591
CCGCGTT1155.0475865E-721.52291149
AAACGCG904.000254E-521.38814719
TAACCGT1701.2551027E-1021.03093153
AACCGAT6950.020.57537734
ACCGATC7200.020.2441635
CAACGAG3150.020.0784915
ACTCTCG1802.7102942E-1019.8611321
CGCGCGT700.004979396219.64217422
CCGCTAG1008.9977846E-519.24950425
AGAGTAC100050.019.18508311
TATCAAC161500.018.6304952
CGCGTTA1352.3487373E-618.33433350
CAACGCA163200.018.1274265
Produced by FastQC (version 0.11.5)