FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159261
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTC43840.13876662928450673No Hit
ATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAGGCATACTCATC37200.11774905587097742No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT32150.10176430500677217No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTTGCTTTTCCTCCGCAA31630.10011835046233913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20800.041.4847072
GTGGTAT22400.037.0063551
GGTATCA45600.032.1620671
GTATCAA73600.031.5936071
GTCTAAC1750.031.5787531
GTCGAGG13850.031.2974878
AGTCGAG17850.030.2364967
TGAGTCG1054.5295837E-628.3318377
TATCAAC93150.027.790082
ATCAACG93400.027.6242353
CAACGCA93900.027.4771425
CCTAGTA3100.027.4235482
TACTAGG2700.026.763352
TCAACGC98800.026.1144084
GAACCGT1001.05550935E-425.5034946
CCGTCGT951.4424586E-924.6051346-47
AACGCAG109400.023.9726646
TATACAG6750.023.9292075
CTAACCG2900.023.4514926
AGAGTAC60550.023.26691410-11
Produced by FastQC (version 0.11.5)