FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159261
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135440.42870785288078445No Hit
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTCATCCAATCCAA43670.13822852876036518No Hit
ATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAGGCATACTCATCTTTTTCAGTGG33910.10733522808023775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20700.031.2300642
GTGGTAT21450.030.7964131
TAGTCGA400.00787838127.49771122
GGTATCA48500.025.9920161
GTCTAAC1600.025.8040281
GAATCGG759.765779E-625.66453222
GCTAGAC1409.094947E-1225.5364250
CCCGTTG1409.094947E-1225.53439912
CGACGTC901.4480538E-624.4451251
TATCGGT901.4490524E-624.44318613
CGAGCGC801.6096357E-524.06202310
TGCGCGA801.6100304E-524.0612612
GAACCGT801.6127959E-524.0559276
GTCGCAA701.7908412E-423.5922531
CGACGGG701.8012559E-423.5720852
GTATCAA82150.023.453451
ATCGGTC952.3194752E-623.15633414
TAAGCCG3100.023.0662536
TCGGTCG600.00203561122.91802641
TTACTCG1203.005698E-822.91584819
Produced by FastQC (version 0.11.5)