FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5492085
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56470.10282069560103313No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55560.10116376567369223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT34950.044.149861
GGTATCA73700.040.7199441
TGGTATC39300.039.4764982
GTATCAA129850.034.4057241
TATCAAC159900.029.9580632
CAACGCA165850.028.3960975
AACGCAG171250.027.6744256
ATCAACG187500.025.548473
TCAACGC191050.025.0287884
CGCAGAG192700.024.413898
ACGCAGA195350.024.1921397
GTCGAGG29000.024.180048
AAGTACG11750.023.5150221
TCGAGGT29750.022.8562039
AGTCGAG37600.022.493047
CAGAGTA121900.021.96382110-11
GCAGAGT217050.021.8316299
CCGCGGT1000.003157913521.2491269
CGCGATA804.891588E-621.2490314-15
AGAGTAC113400.020.96805410-11
Produced by FastQC (version 0.11.5)