FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353556
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39771.1248571654843929No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12370.3498738530812658No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6890.19487719060064035No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.13265225310841847No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4260.12049010623493873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCGA150.005776896655.0082140
GTGGTAT9150.045.127021
TGGTATC9150.045.075942
ACGGGTA401.8988026E-434.37526716
CGAGCCC350.004092834431.43326438
CGTGTTC350.004092834431.43326437
CGGGTAA350.004095671731.42881417
GTATCAA36600.027.5274831
TAACAGT507.0277136E-427.496324
GCCGATC400.00787101727.49631941
CGAGGAC400.00787101727.4963193
TTAAACG759.726928E-625.66686422
GATACCA1409.094947E-1225.53952811
GCTCGCT651.08786364E-425.38121830
ATACCAC1351.5825208E-1024.44463312
TATCAAC41850.024.2440682
GGTATCA17850.024.184443
TCGAGGG2550.023.7290339
CGAGGGT2350.023.4077510
TATTAGC600.002034340322.9135992
Produced by FastQC (version 0.11.5)