FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746179
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT42270.5664860576349642No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32680.4379646170690947No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.3189583196525231No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC23190.3107833375101685No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA22850.306226790086561No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC20960.2808977470553312No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA19180.2570428811317392No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT18560.24873388288869028No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA17100.229167532187317No Hit
GAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGA16740.22434295256232084No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.21147740689566444No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT15560.20852905268038902No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC14690.19686965191998165No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA12510.1676541419686161No Hit
GTCTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAG12270.16443775555195203No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT12240.16403570724986902No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.16282956234361998No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11550.1547885963019597No Hit
TGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTG11340.15197425818737864No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.15103614548251826No Hit
GTCTTCTTCAGTTTCGACTTATCGAATTTCTCGATCTCAGCCATATCGGG10680.14312919554155237No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT10620.14232509893738635No Hit
GTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTG10530.1411189540311373No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10500.1407169057290543No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.1364283905068355No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT9880.13240790748600537No Hit
GGGCAGCACAGTCATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAG9810.131469794781145No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.13133577868045068No Hit
GTAATGAGGCGTGCGCCGCCAATATGCACTGTACATTCCACAAGCATTGC9700.12999561767350729No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA9690.12986160157281296No Hit
TAGATAGACAGATGGGAAAGGCAGGCGCGGCCTTCGTTGTCAGTAGTTCT9640.12919152106934126No Hit
CATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAGTTAAACAGCTAA9160.12275874823601308No Hit
GTTTAACTTTGTAAGATGCAAAGAGGTTGGATCAAGTTTAAATGACTGTG8970.12021244232282066No Hit
CTGATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTT8860.11873826521518295No Hit
GGAGAAGCAAGCAGGCGAATCGTAATGAGGCGTGCGCCGCCAATATGCAC8750.11726408810754524No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTC8570.11485179829504716No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGC8520.11418171779157549No Hit
ATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAG8300.11123336357630005No Hit
GTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGA8190.10975918646866235No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT8130.10895508986449633No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7780.10426452634019451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)