FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624093
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT25490.40843271755972266No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.24691832787741572No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14860.23810553875784538No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14240.22817112193214795No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.14645253191431404No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8860.14196602108980552No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.14068416085423166No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8410.13475555726470254No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7980.12786555849849301No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.10302951643424939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG1500.053.848461
ATATCAA9050.052.6114651
TATACGG250.007785938751.014332
TGGTATC10300.050.7666932
GTGGTAT9700.049.962491
AAGTACG3900.047.962191
TATCAAC49350.037.7309652
GTATCAA38250.036.7881131
GTCGAGG10800.035.8058978
TCGAGGT10300.035.068629
GGTATCA19300.035.0227931
GACCGTG500.002788555633.9986537
ATCAACG58750.032.2728963
TCAACGC59300.031.9735724
CAACGCA58300.031.8657265
AGTCGAG14650.031.6199057
GCGTCGG550.004449071430.9053929
AACGCAG60450.030.8002286
AGTATCA4150.030.7315251
GCACCGT703.9421095E-430.355947
Produced by FastQC (version 0.11.5)