FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624093
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67111.0753205051170258No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16510.2645439061165563No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10730.1719295040963446No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6620.10607393449373731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10750.039.681351
TGGTATC11250.037.899492
GTGCGAA350.004095813731.43232512
GTATCAA47000.028.2235981
CATATGT400.00787198827.4988774
GACGACG400.00787198827.49887726
ACGACGC400.00787198827.49887727
ACGGGAT904.7215508E-827.4944716
ACGGGGC1057.2614057E-926.18521116
TATCAAC52550.025.4400392
GGTATCA22000.025.0190331
ATAAACG801.6022277E-524.0711633
AGAGTAC34850.022.88652411
CAGAGTA36150.022.36781910
ATCAACG60450.021.7530753
TCAACGC61000.021.54834
CAACGCA61450.021.3887885
CGGGTAA903.998056E-521.38458817
TTAAACG1306.9845555E-821.1546822
CGGGATT650.003241888721.14959317
Produced by FastQC (version 0.11.5)