FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852830
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT88190.475974590221445No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56050.30251021410490975No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52340.2824867904772699No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39120.21113647771247226No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.17087374448816137No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31120.1679592839062407No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.15014869146117021No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23460.126617120836774No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22470.12127394310325287No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22310.12041039922712823No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.10756518406977433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG5700.067.116171
AAGTACG10900.059.2756921
GTGGTAT31650.055.332851
TGGTATC35150.053.8109632
ATATCAA28400.040.561171
GTATCAA121300.038.6872441
TATCAAC153000.038.1985932
ATCAACG173850.033.6173973
TCAACGC177600.032.8836364
CAACGCA179850.032.4958845
GGTATCA61950.032.0414123
GTCGAGG30350.031.925818
ACGGGTC802.3062434E-531.8784625
AGTATCA10850.031.3415031
AACGCAG188250.031.1587626
TCGAGGT30200.030.6771729
AGTCGAG40150.028.896347
TCGCTAG300.006807486528.33335326-27
CGCAGAG218650.026.6473318
CGCTAGT408.61505E-426.56251726-27
Produced by FastQC (version 0.11.5)