FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852830
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208041.1228229249310515No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43620.2354236492284775No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28330.1529012375663175No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19870.10724135511622761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT37900.041.2466051
TGGTATC39050.039.79362
TTCGCTA300.001926813436.66322718
TGTCGAC350.004097822631.43241346
GTATCGG507.00084E-427.5219421
GTATCAA147150.026.5213151
TATCGGC550.001225964325.0030572
AGAGTAC95050.024.04165511
TATCAAC164650.023.8869152
CAGAGTA97800.023.39375510
TTAAACG4050.023.08612422
GGTATCA76200.022.9710711
ATCAACG181650.021.423733
TCAACGC185400.020.9567744
CAACGCA187050.020.8137635
AACGCAG192900.020.3371436
TCGCAAC700.004978305719.64207629
AGTACGG115500.019.5468413
ACAACGT1009.003935E-519.2466378
GAGTACG117750.019.19668812
Produced by FastQC (version 0.11.5)