FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114851
Sequences flagged as poor quality0
Sequence length91
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT79546.925494771486535No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11991.0439613063882769No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT10980.9560212797450609RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA9190.8001671731199554No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC7960.6930718931485141No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.6599855464906705No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT5720.49803658653385696RNA PCR Primer, Index 29 (96% over 25bp)
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.49629519986765464No Hit
ATATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA4590.3996482398934271No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGG4290.37352743990039267No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.36569119990248233No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG4000.34827733324045934No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT3890.33869970657634674No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAA3880.3378290132432456No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.2821046399247721No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAA3220.2803632532585698No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA3180.2768804799261652No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.2629493865965468No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.24292343993522042No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGT2780.24205274660211926No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA2440.21244917327668023No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.20548362661187103No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.20025946661326416No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.1793628266188366No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGT2030.17675074661953313No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.1689145066216228No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.1671731199554205No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGCCGT1910.16630242662231937No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA1780.15498341329200443No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.14540578662789178No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC1660.14453509329479064No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1590.1384402399630826No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.1314746932982734No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGC1510.1314746932982734No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT1450.12625053329966654No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.12625053329966654No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCT1420.12363845330036306No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.12189706663416078No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT1290.11231943997004816No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.11231943997004816No Hit
AGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAA1280.11144874663694701No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT1270.11057805330384585RNA PCR Primer, Index 29 (95% over 22bp)
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC1150.10012973330663208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG550.077.309971
ATATCAA1650.074.732961
ATACGGG650.071.957731
AGTATCA800.063.7807241
CTGAGAC200.003215505763.752949
TCTGAGA302.2461492E-456.6692778
ATATAAG250.00776368751.0245741
GTATTGA250.00777707751.002358
ACATTGC250.00777707751.002358
GCTCTTA250.00777707751.002357
CAGCTCT354.7870047E-448.6160165
AAGCTAA354.8076402E-448.573679
AGGAAGC409.2424615E-442.5390176
GAAGCTA409.2822243E-442.501968
TAATCGG209.301694E-442.5019634-35
ACGGGAT255.4508135E-542.48345616-17
TGGTATC4950.042.0909772
GTGGTAT4300.041.531631
GTATCAA21700.040.9529041
TCGAGGT4600.038.8061379
Produced by FastQC (version 0.11.5)