FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114851
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1870516.28631879565698No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11661.015228426395939No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.2960357332543905No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.2098370932773768No Hit
TATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.20374223994566876No Hit
ATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.18458698661744347No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC1620.14105231996238604No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.12102637330105963No Hit
GAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.11057805330384585No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA1230.10709527997144125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTGT150.005743133455.0658531
ATATAAC150.005743133455.0658531
TATAACC150.005753055755.0418472
ATGTGTA150.005753055755.0418472
TTCAAAT257.814347E-444.01429412
GTGGTAT5100.036.7105671
TCAAATT300.001915361336.67857713
TCAGAGT300.001915361336.6785779
CCAAGAC300.001915361336.6785773
AACTCAG300.001919465636.66266
ACTCAGA300.001919465636.66267
TGGTATC5350.035.4942782
TGTATAA558.5583997E-735.026632
ACGGGAC658.290044E-833.842416
CGGGGTG601.7009334E-632.07977717
TCGACAT350.00407792531.438784
ATAACCA350.00407792531.438783
CTCAGAG350.004086636531.4250878
CTATCTC350.004086636531.42508746
CGACATT350.004086636531.4250875