FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221225
Sequences flagged as poor quality0
Sequence length61
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91214.122951745959996No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35861.6209741213696462No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20670.9343428636004067No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14900.6735224319132105No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10490.47417787320601196No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9050.40908577240366145No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7370.3331449881342525No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.29472256752175385No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6310.2852299694880778No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5610.25358797604249067No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4810.2174256978189626No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4450.20115267261837494No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.1848796474177873No Hit
GTGGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.1740309639507289No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTT3300.14916939767205334No Hit
GTATCAACGCAGAGTACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.13877274268278902No Hit
ATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.13470448638264212No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2890.1306362300824952No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2770.125211888348966No Hit
GGTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.12249971748220138No Hit
GAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.11436320488190756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATA150.00577294955.0108531
TCGTGCG150.00577294955.01085334
CAGGGTA455.2750693E-1155.0108531
CCGATGA150.00577294955.01085336
TGCGGGT150.00577294955.01085337
TGGCAGC150.00577811854.99841315
GTCATCC150.00577811854.99841313
TAGCCTG150.00577811854.99841310
CATCCAA150.00577811854.99841315
CCAGACT150.00577811854.99841348
CTGGCAG150.00577811854.99841314
TTCCCAA150.00577811854.99841322
GCTCGTG150.00577811854.99841332
TAATCCA150.00577811854.99841344
ATCGTCA150.00577811854.99841310
GCCTGGC150.00577811854.99841312
CTCGTGC150.00577811854.99841333
TCGAGGA150.00577811854.9984139
GTTAATC150.00577811854.99841342
CATCGTC150.00577811854.9984139
Produced by FastQC (version 0.11.5)